Recombinant protein production service
Icosagen provides recombinant protein production services using proprietary CHO-based QMCF Technology in serum-free conditions. Customer benefits are a short production time and cost-efficiency.
We offer two options for protein production:
- Enhanced transient expression of recombinant proteins using high copy number transient pQMCF-T expression vectors. This option is fast, and a good fit for a small-scale pilot study to test the best expression conditions for your protein of interest.
- Production using stable episomal production pools for large-scale production using pQMCF expression vectors that are stably maintained in proliferating CHO cells. This option is scalable and suitable for the cost effective production in up to 40 liter scale, and to produce protein of interest in up to gram-scale.
Transient production of recombinant proteins includes:
- cDNA optimization and cloning into transient pQMCF-T expression vector
- Chemical transfection of QMCF/CHOEBNALT85 cell line and expression/secretion control 48 hours after transfection
- Optional culture expansion (up to 2.5 folds) and recombinant protein production
- Harvesting and protein purification
- Product analysis and characterization
-
Weekly updates, and detailed reports
Stable production including selection and cell bank generation:
- cDNA optimization and cloning into pQMCF expression vector
- Electroporation of QMCF/CHOEBNALT85 cell line and expression/secretion control 48 hours after transfection
- G418 selection and generation of production cell bank (stable episomal pool)
- Culture expansion to the final production volume and recombinant protein production
- Harvesting and protein purification
- Product analysis and characterization
-
Weekly updates, and detailed reports
| Process Description | Deliverables |
| cDNA optimization and cloning into pQMCF
expression vector | Sequence data,
construction information |
| Transfection and antibiotic selection (in the case of stable production) for
enrichment of protein production cell population |
Expression/secretion data at 48 hours after transfection
|
| Generation of production cell bank (not applicable in transient production format) | At least 2 vials of the cell pool expressing protein of interest |
| Recombinant protein production | Protein-containing
cell culture medium.
Production analysis data (WB, ELISA, Coomassie-stained SDS-PAGE, Octet K2)
|
| Purification
optimization and purification | Purification protocol, and purified protein (>95% purity; less than 2% aggregates) |
| Product
characterization | Project report including QC data (WB, ELISA, Coomassie-stained SDS-PAGE, analytical SEC, binding and kinetics analysis by Octet K2) |
Produced recombinant proteins are suitable for various types of pre-clinical investigations: drug discovery, antibody generation, structural biology, functional characterization, development of analytical and diagnostic tools.