Proteins

SARS-CoV-2 Trimeric Spike Californian B.1.427

P-354-100

Datasheet as
Name SARS-CoV-2 Trimeric Spike Californian B.1.427
Description Protein contains amino acids 14-1211 and following mutations: W152C, L452R, D614G. Plus two extra amino acids (AS) in N-terminus, trimerization domain and His-6 tag at C-terminus, GS linker between protein and trimerization domain and GSG linker between trimerization domain and His-tag. Furin cleavage (RRAR) site between Spike S1 and S2 is mutated (GSAS).
Uniprot ID P0DTC2
Source Human
MW 410.645 kDa
Host CHO-based cell line (expressed by QMCF Technology)
Purification Purified by Ni-affinity chromatography and gel-filtration from serum-free CHO growth media, sterile filtrated
Purity >95%
Concentration 1 mg/ml
Buffer PBS pH 7.4
Endotoxine NA
QC SDS-PAGE, analytical SEC, Octet binding
Shipping Shipped on dry ice.
Storage Store at -20 °C to -70 °C long term. Avoid multiple freeze-thaw cycles.
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€750 / 100µg
€1450 / 500µg
€1950 / 1000µg

Figure 1.  Simply Blue stained SDS-PAGE analysis of SARS-CoV-2 Trimeric Spike Californian B.1.427. 4-12% gradient gel is used for analysis. Lane 1. 0.8 µg SARS-CoV-2 Trimeric Spike Californian B.1.427 (-DTT) Lane 2. Protein marker (Smobio) Lane 3. 0.8 µg SARS-CoV-2 Trimeric Spike Californian B.1.427 (+DTT)
Figure 2. Octet Red96e analysis of SARS-CoV-2 Trimeric Spike Californian B.1.427 binding to human ACE2 receptor.