Proteins

SARS-CoV-2 Spike RBD2 UK VOC 202012/01 (alpha)

P-311-100

Datasheet as
Name SARS-CoV-2 Spike RBD2 UK VOC 202012/01
Source cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1)  Protein contains amino acids 319-541, mutation N501Y, plus two extra amino acids (YS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.
MW 26,33 kDa
Host CHO-based cell line (expressed by QMCF Technology)
Purification Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity > 95%
Concentration 1 mg/ml
Buffer PBS pH 7.4
Endotoxine NA
QC SDS-PAGE
NanoDrop A280
analytical SEC
Background Measured by its binding ability to ACE2 protein by OCTET RED96 system.
Shipping Shipped on dry ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
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Figure 1. SDS-PAGE analysis in reduced and non-reduced conditions. 1.3 µg of SARS-CoV-2 Spike RBD2 UK VOC 202012/01 protein were loaded per lane. Lane 1.
SARS-CoV-2 Spike RBD2 UK VOC 202012/01 (reduced); Lane 2. Protein size marker (Smobio); Lane 3. SARS-CoV-2 Spike RBD2 UK VOC 202012/01 (non-reduced)

Figure 2. Octet Red96e analysis of SARS-CoV-2 Spike RBD2 UK VOC 202012/01 binding to human ACE2 receptor.