Proteins

SARS-CoV-2 Spike RBD2 Brazil P1 (gamma)

P-315-100

Datasheet as
Name SARS-CoV-2 Spike RBD2 Brazil P1
Source cDNA encodes SARS-CoV-2 surface protein (YP_009724390.1)

Protein contains amino acids 319-541, mutations K417T, E484K, N501Y, plus two extra amino acids (YS) in N-terminus and His-6 tag at C-terminus and GSG linker between protein and tag.
MW 28.4 kDa
Host CHO-based cell line (expressed by QMCF Technology)
Purification Metal-affinity chromatography following gel filtration. Protein is sterile-filtrated through 0.22 µm filter.
Purity >95%
Concentration 1 mg/ml
Buffer PBS pH 7.4
Endotoxine NA
QC Coomassie stained SDS-PAGE, analytical SEC
Shipping Shipped on dry ice
Storage Store at -70°C upon receipt. Recommended to aliquot into smaller quantities. Avoid repeated freeze-thaw cycles
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€500 / 100µg
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Figure 1. Coomassie-stained SDS-PAGE analysis of SARS-CoV-2 Spike RBD2 Brazil P1. 4-15% gradient gel is used for analysis.  Lane 1. 1.3 µg SARS-CoV-2 Spike RBD2 Brazil P1 (+DTT) Lane 2. Protein marker (Smobio) Lane 3. 1.3 µg SARS-CoV-2 Spike RBD2 Brazil P1 (+DTT)

Figure 2.  Octet Red96e analysis of SARS-CoV-2 Spike RBD2 Brazil P1 protein binding to human ACE2 receptor.